How To Use Cosmid In A Sentence
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During further analysis of these cosmids, high throughput sequence databanks were periodically screened for homology to the unique sequences until unannotated files containing exact matches appeared.
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These gap sizes were reduced, but not eliminated, by shearing the linear cosmid into smaller fragments.
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1987 (Burke et al. 1987) permitted much larger segments of DNA to be ordered and stored for sequencing than was possible with plasmid or cosmid libraries.
The Human Genome Project
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E. coli and 25 µg/ml for M. smegmatis; and hygromycin B, 100 µg/ml for oppA (Rv1280c) of M. tuberculosis was amplified from cosmid MTCY50 using the primer pair
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The alphaT2ib open reading frame was integrated into an adenoviral cosmid vector for production of recombinant adenovirus (AdV) - alphaT2ib.
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Lane 1 corresponds to a cosmid from human chromosome 6 and serves as a control.
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Hybridisation of the cosmid library representing a 4 fold coverage of human chromosome 21 with the exon-enriched probe.
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A chromosome-specific cosmid library was screened with the amplified 240-bp npkA fragment identified in the differential display-reverse transcriptase polymerase chain reaction procedure.
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With porcine myoglobin genomic DNA fragment cloned in a cosmid as probe, the chromosome location of this gene was determined by fluorescent in situ hybridization (FISH).
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Hybridisation of the cosmid library representing a 4 fold coverage of human chromosome 21 with the exon-enriched probe.
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However, the linearized cosmid produced an incomplete assembly because the two DNA termini, though greatly over-represented in the clone library used for sequencing, were separated from neighboring sequences by gaps of ~1. 4 and 1.8 kb.
BioMed Central - Latest articles
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Leda Cosmides and John Tooby have produced an excellent primer on the subject so we will use that as a point of reference throughout this post.
