How To Use Counterstain In A Sentence
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The sections were then counterstained in eosin, dehydrated, cleared, and mounted with glass coverslips.
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Following incubation with fluorescence-conjugated secondary antibody (at room temperature for 60 min) and rinses, slides were incubated with DAPI (1: 10,000, Sigma, St. Louis, MO) for 15 minutes to counterstain nuclei, mounted, and cover-slipped.
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The chromosomes are counterstained with DAPI (light blue).
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Harris hematoxylin was the counterstain for the remainder of the antibody reactions.
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After washing in tap water, specimens were briefly counterstained.
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α (brown; g; nuclear counterstain is methyl green), Ttf1
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The sections were counterstained with hematoxylin and eosin.
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Bottom: reelin immunofluorescence (red) overlaid with MAP2 counterstain
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After washing, the HRP (horseradish peroxidase) conjugated polymer complex was visualized by incubation for ~5 min with AEC (3-amino-9-ethylcarbazole) substrate-chromogen; sections were then counterstained with hematoxylin for 30 seconds, washed and mounted.
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The shape of the nucleus was visualized using a DNA counterstain in all experiments.
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Immunoreactivity was visualized using diaminobenzidine as the chromogen, with Harris modified hematoxylin as the counterstain.
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Background material in the specimen was counterstained dull yellow to red.
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Slides were counterstained with hematoxylin, dehydrated, and mounted with glass coverslips.
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The sections were then counterstained in eosin, dehydrated, cleared, and mounted with glass coverslips.
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Sections were counterstained with hematoxylin and mounted in permanent mounting medium.
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No counterstain was used on the sections, but routine coverslips were applied.
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Stain section of kidney tissue provided (showing abscess formation by Staphylococcus aureus) by Gram's method, and counterstain with cosin.
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Anti-calbindin antibody labels Purkinje cell bodies and dendritic arbors, while the Nissl counterstain (purple) labels all neuronal cell bodies.
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Dapi was used as the chromosome counterstain for fluorescent specimens.
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Slides were counterstained with hematoxylin, dehydrated, and mounted with glass coverslips.
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Detection was completed using HRP-mediated hydrogen peroxide immunostaining (AEC+, DAKO), with hematoxylin as a counterstain.
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Following PBS wash, slides were counterstained with a Hoescht counterstain (Sigma B2883).
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Acridine orange (Citrus sinensis) fluorescent counterstain auramine acid-fast stain
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Following development of in situ signal, sections were counterstained with anti-K5 and anti-K8 antibodies followed by fluorochrome conjugated secondary reagents to allow co-localization of Cells were suspended in 100 µl of FACS staining buffer (FSB-1% fetal bovine serum, 5 mM EDTA and 0. 02% NaN 3 in PBS) with appropriately diluted primary antibodies for 20 minutes on ice in the dark.
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The chromosomes were counterstained with propidium iodide.
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Slides were counterstained with hematoxylin and mounted.
