How To Use Polymerase In A Sentence
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Details of the polymerase chain reaction are given in a previous publication.
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The more drugs that one gives, the more likely it is that damage to host DNA polymerase will take place.
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Presence of oncogenic HPV types 16 and 18 DNA was done by polymerase chain reaction.
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“A polynucleotide system based on a ribozyme polymerase able sequentially to add the correct nucleotides and thus copy itself might work.”
Good Math, Bad Math, and David Berlinkski - The Panda's Thumb
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However, a nasopharyngeal aspirate is the best specimen for detecting influenza viruses, and polymerase chain reaction is more sensitive than tissue culture when serology is the reference standard.
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To allow for the next round of transcription initiation RNA polymerase has to translocate about 60 base pairs.
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Acyclovir, an acyclic guanosine analog, binds viral DNA polymerase, acting as a chain terminator and ending replication.
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In 1969 – 70, the isolation of an RNA-polymerase associated with the viral particles of the vesicular stomatitis virus led to the idea that perhaps a key enzyme was also associated with the oncogenic RNA viruses.
Luc Montagnier - Autobiography
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Details of the polymerase chain reaction are given in a previous publication.
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Replicative DNA polymerases are very accurate enzymes with error rates usually in the range of 10-5 per replicated nucleotide.
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The procedure was based on a method called polymerase chain reaction.
SPIX'S MACAW: THE RACE TO SAVE THE WORLD'S RAREST BIRD
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Such a structural change in the initiation complex can result from direct contacts between the transcription factor and RNA polymerase.
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Methods To illustrate the relationship of H3 subtype influenza virus with other subtypes, we carried out the sequence analysis of PB1 polymerase genes of three H3N8 subtype du.
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METHODS:Polymerase chain reaction restriction fragment length polymorphism(PCR RFLP) was used to analyze NQO1 gene polymorphism in Parkinson disease group and healthy adult control group.
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In particular, work on vesicular stomatitis viruses has shown that the phosphoprotein, or P protein, interacts directly with the polymerase and nucleoproteins and plays a role in transcription and replication.
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The gap is then filled in by a DNA polymerase and the new sequence ligated with the flanking regions.
Another Protozoan and Front-Loading
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Method Polymerase chain reaction and restriction fragment length polymorphism were used to examine 40 oral malignant tumors for loss of heterozygosity at chromosome 3p.
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Methods We used the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) to examine 40 oral squamous cell carcinomas for loss of heterozygosity (LOH) at APC.
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Also present in the nucleoplasm are proteins known as polymerases, which pluck nucleotides from the soup as needed when copying DNA.
The Speculist: Better All The Time #22
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Both topoisomerase I and RNA polymerase II reactivity occurs in the minor groove of DNA.
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A polymerase chain reaction ( PCR ) was developed and evaluated to detect porcine parvovirus ( PPV ).
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Biochemical reaction, coagglutination test, metabolism inhibition test, polymerase chain reaction (PCR) assay, and DNA sequencing were employed to identify the isolated microorganisms.
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The P protein associates with monomeric N protein destined for nucleocapsid assembly and serves as a polymerase co-factor essential for RNA synthesis
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These complexes regulate the interaction of RNA polymerases and DNA elements within promoters.
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The growing RNA chain still attached to the polymerase is a target for translating ribosomes or polyribosomes which are engaged in the synthesis of proteins.
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In contrast to the phage polymerases, RNA polymerases from higher organisms have a much more complex subunit structure than the bacterial enzymes.
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Subsequently, recombination was also shown to be stimulated by RNA polymerase II-driven transcription.
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In particular, work on vesicular stomatitis viruses has shown that the phosphoprotein, or P protein, interacts directly with the polymerase and nucleoproteins and plays a role in transcription and replication.
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The DNA samples were denatured after irradiation, a primer was annealed to one of the strands, and a complementary strand was constructed using a polymerase enzyme.
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Objective To develop a novel multiplex polymerase chain reaction ( PCR ) to detect multidrug - resistant Acinetobacter baumannii.
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You cite an example of defects in polymerase proofreading and mismatch repair leading to an increased risk of cancer.
The Memory Hole
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In reactivated lesions epithelial syncytia and inclusion bodies were not seen; however, virus was demonstrable by polymerase chain reaction and culture.
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Method Polymerase chain reaction and restriction fragment length polymorphism were used to examine 40 oral malignant tumors for loss of heterozygosity at chromosome 3p.
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Processivity is defined as the number of deoxyribonucleotides incorporated each time a DNA polymerase binds its template-primer.
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The more drugs that one gives, the more likely it is that damage to host DNA polymerase will take place.
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In addition, polymerases generally prefer unmodified nucleotides to modified ones, thus creating a probe that is only lightly labeled.
The Scientist
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A thermostable DNA polymerase is added to the same tube.
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Promoter sequences promote the ability of RNA polymerases to recognize the nucleotide at which initiation begins.
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Polymerase chain reaction, however, is a powerful technique capable of amplifying specific target DNA sequences by a factor of 10.
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These DNA-binding proteins include polymerases, helicases, nucleases, isomerases, ligases, histones, and others.
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Isada NB, Parr DP, Johnson MP, Straus SE, Holzgreve W, Qureshi F, Evans MI: In utero diagnosis of congenital varicella-zoster virus (VZV) infection by chorionic villus sampling (CVS) using polymerase chain reaction (PCR).
Prenatal Diagnosis
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To prevent oxidation of polyphenol and its interference with activity of Taq polymerase; 2-mercaptoethanol was added to an extraction buffer and insoluble polyvinylpyrrolidone was added to leaves when they were ground.
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To address the foregoing question, we applied the arbitrary primed polymerase chain reaction technique.
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According to the RNA polymerase partition model two interconvertible populations of RNA polymerase exist in the cell.
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The assembled factors include an RNA polymerase, the blue molecule.
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The TPH gene A218C polymorphism and the MAOA gene T1460C polymorphism were determined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique.
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Nobel Prizes have recognized a number of the triumphs along the way, among them Watson, Crick and Wilkins 'decipherment of the helical structure of DNA and Roger Kornberg's uncovering of the workings of the enzyme RNA polymerase, which turns DNA into RNA.
The Nobel Prize in Chemistry 2009 - Speed Read
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The chemistry of water activated by a magnesium ion is central to the function of the DNA repair proteins, apurinic/apyrimidic endonuclease 1 (Ape1) and polymerase A (Pol A).
Signs of the Times
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A key part of Pacific Biosciences 'system is an enzyme called polymerase that human cells use to copy DNA.
Intel, Others Back New DNA Sequencer
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A polymerase chain reaction analysis for simian virus 40 DNA was negative.
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Method The histochemical staining and polymerase chain reaction was used to detect oral leukoplakia.
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These results are in keeping with our analysis of the polymerase and DNA binding assay for this mutant.
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In the phylogenetic tree of TERT and retroelements rooted with RNA-dependent RNA polymerases, the RT motifs in the RT family are universally conserved [including multicopy single-stranded DNA, group II introns, LTR or non-LTR (Long Terminal Repeat) retrotransposons, and viral RT] [10], [89].
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Sampling occurs when the extract is pipetted into the PCR mix and again when the primers and polymerase bind the template DNA in the PCR.
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I'm not sure what you are referring to by 'known mycobacterial mutations', but the main polymerase responsible for induced mutagenesis in M. tuberculosis including antibiotic resistance following infection (DnaE2 – see Boshoff et al Cell.
Browsing the Latest Issue of Scientific American
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A chromosome-specific cosmid library was screened with the amplified 240-bp npkA fragment identified in the differential display-reverse transcriptase polymerase chain reaction procedure.
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A multidisciplinary international team demonstrated through botanical examination, phytochemical investigation, and genetic deoxyribonucleic acid analysis by polymerase chain reaction that this material contained tetrahydrocannabinol, the psychoactive component of cannabis, its oxidative degradation product, cannabinol, other metabolites, and its synthetic enzyme, tetrahydrocannabinolic acid synthase, as well as a novel genetic variant with two single nucleotide polymorphisms.
2,700-year-old marijuana stash found
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The presence of the histones and CpG methylation most affect accessibility of the chromatin to RNA polymerases and transcription factors.
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A thermostable DNA polymerase is added to the same tube.
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A mutagenic oligo containing one of the mutations was annealed to a genome carrying the second mutation, and the oligo was extended using T4 DNA polymerase, essentially as in Sambrook, Fritsch, and Maniatis.
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And it goes through a process called PCR, polymerase chain reaction.
CNN Transcript Apr 22, 2008
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Methods: Polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) was used to exclude known gene mutation of HGF from 2 familial gingival fibromatosis .
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Motors, such as RNA polymerases and ribosomes, produce trailing strands of mRNA and protein, respectively.
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During its path along the template, RNA polymerase may encounter positions where it hesitates and pauses for a variable period of time.
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Researchers believe that the bird virus polymerase allows for faster replication, and thus more aggressive.
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The specialized techniques such as immunocytochemistry and polymerase chain reaction are also used with slightly more promising results.
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Infection is diagnosed more accurately by serum antibody tests or molecular polymerase chain reactions.
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The vectors were linearized and transcribed with a DNA-dependent RNA polymerase.
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Methods LOH at APC and MCC genetic loci in 46 specimens resected from esophageal neoplasm was studied with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).
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DNA molecule to which RNA polymerase binds, initiating the transcription of messenger RNA.
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Reverse transcriptase, polymerase chain reaction, nucleic acid probe test---the whole nine yards.
MINUTES TO BURN
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DNA polymerase, Pol I, extends the DNA from the free 3'-OH using its exonuclease activity to replace the nucleotide of the damaged base, as well as a few downstream, followed by sealing of the new DNA strand by DNA ligase.
Another Protozoan and Front-Loading
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These DNA-binding proteins include polymerases, helicases, nucleases, isomerases, ligases, histones, and others.
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However, additional studies are needed to determine if Twin is actually transcribed by polymerase III.
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Semi - quantitative reverse transcription - polymerase chain reaction ( RT - PCR ) was used to detect the level of MMP - 12 mRNA expression.
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The polymerase chain reaction assay, branched DNA assay and nucleic acid sequence-based amplification assay quantitate human immunodeficiency virus RNA levels.
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Kamphaeng Phet Province; MoPH, Ministry of Public Health; nhD, nonhospitalized dengue; PCR, reverse transcriptase-polymerase chain reaction
PLoS Medicine: New Articles
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I'm not sure what you are referring to by 'known mycobacterial mutations', but the main polymerase responsible for induced mutagenesis in M. tuberculosis including antibiotic resistance following infection
Browsing the Latest Issue of Scientific American
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Researchers believe that the bird virus polymerase allows for faster replication, and thus more aggressive.
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The primase utilizes the DNA strands as templates and synthesizes a short stretch of RNA generating a primer for DNA polymerase.
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Objective Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) assay was applied to molecular typing of resistance-associated esterase genes of Culex pipiens complex.
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Isolates underwent polyphasic analyses employing phenotypic (selective media and biochemical testing) and genotypic (polymerase chain reaction) assays to determine species identification.
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A protein complex called the polymerase, which is attached at the end of RNA, helps kick off replication once the assembly is injected into a healthy cell.
Health News from Medical News Today
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A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II.
Roger D. Kornberg - Autobiography
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METHODS:Polymerase chain reaction restriction fragment length polymorphism(PCR RFLP) was used to analyze NQO1 gene polymorphism in Parkinson disease group and healthy adult control group.
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Just the inducibility of less accurate DNA polymerases (among some other genes).
Browsing the Latest Issue of Scientific American
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We analyzed gene mutations in patients who died of thrombotic events, using multiplex allele-specific polymerase chain reaction amplification.
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Genotypes were obtained by automated sizing of fluorescently tagged polymerase chain reaction amplification products.
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RNA polymerase binding may be inhibited by the distorted promoter structure.
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We used the following key words: cancer, tumour markers, prognosis, various malignant diseases, and RT-PCR [reversed transcriptase and polymerase chain reaction].
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Genes were amplified from genomic yeast DNA using specific oligonucleotides and the polymerase chain reaction.
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The experiment I referenced demonstrated (actually a series of experiments) that DNA damage in M. tuberculosis results in the induction of an SOS-regulated polymerase (DNAE2), which causes base substitution mutagenesis, that is, the experiment was set up so that the only drug resistant mutations that was scored was base substitution mutagenesis.
Browsing the Latest Issue of Scientific American
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METHODS:Polymerase chain reaction(PCR) combined with restriction enzyme digest ion were used to detect gene specific junction fragments of the 23 CMT1 patients and 30 normal controls.
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Immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) were employed to detect the protein and mRNA expression of RECK and MMP-2 in keratocystic odontogenic tumor (KCOT), ameloblastoma and ameloblastic carcinoma.
BioMed Central - Latest articles
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Immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) were employed to detect the protein and mRNA expression of RECK and MMP-2 in keratocystic odontogenic tumor (KCOT), ameloblastoma and ameloblastic carcinoma.
BioMed Central - Latest articles
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During DNA replication, DNA polymerase incorporates unmethylated cytosine opposite to guanine, leading to an overall reduction in methylation of DNA.
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These newer polymerases have been isolated from a diverse variety of thermophilic organisms and show different enzymatic properties.
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Thus transcribing RNA polymerases and ribosomes may all be bound to the same RNA molecule while marching in the same direction.
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It was a stretch of DNA that could be transcribed by an enzyme called polymerase into a chemically similar molecule known as RNA.
The Economist: Correspondent's diary
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Thus, two of the polymerase mutants are modest spontaneous mutators for some point mutations in yeast strains that are proficient in DNA mismatch repair.
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Telomerase is RNA dependent DNA polymerase, acts to synthesize DNA repeat fragment of telomeres , which is associated with immortalization of cells.
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The ability of DNA polymerases to replicate DNA requires a number of additional accessory proteins.
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First the DNA is transcribed into messenger RNA by the enzyme RNA polymerase; then the messenger RNA is translated into protein by ribosomes.
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Each of these nine rudimentary polymerases is a potentially promising evolutionary intermediate between ligase and replicase.
Good Math, Bad Math, and David Berlinkski - The Panda's Thumb
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The first step involves using a device called a polymerase chain reaction instrument to measure the levels of an organism's cytokines when exposed to a given material.
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In 1969 – 70, the isolation of an RNA-polymerase associated with the viral particles of the vesicular stomatitis virus led to the idea that perhaps a key enzyme was also associated with the oncogenic RNA viruses.
Luc Montagnier - Autobiography
-
As part of its work, the research team used a highly sensitive technique that relied on something called a polymerase chain reaction PCR.
The Panic Virus
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A protein binding to the promoter region could cause steric occlusion and prevent RNA polymerase from initiating transcription.
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Reverse transcriptase, polymerase chain reaction, nucleic acid probe test---the whole nine yards.
MINUTES TO BURN
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The RNA polymerase binds to DNA with the help of other proteins called transcription factors.
