How To Use Protease In A Sentence
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A protein resistant to heat and proteolysis that inhibits serine proteases was isolated from wheat leaf apoplasts.
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The viral nucleocapsid enters the host cell and breaks open releasing two viral RNA-strands and 3 essential replication enzymes: Integrase, Protease and Reverse Transcriptase
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Some bacteria naturally have more of these protease and peptidase enzymes than other bacteria, so careful characterization is necessary to select the strains with the highest level of the desired enzymes.
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Leukocytes may adhere, transmigrate, release proteases, cause additional endothelial injury, and lead to the development of inflammatory changes in the vessel wall.
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This category contains phospholipids, proteolytic enzymes, proteinases, proteases, and peptidases.
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During normal catabolism, protease enzymes break down carboxylase enzymes and reclaim the associated amino acids and biocytin for recycling.
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You need a supplement that provides all of the three main groups of enzymes - amylase, protease and lipase - to break down fats, carbohydrates and proteins.
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The invention discloses a composite stabilizing agent for preserving a liquid acid protease, which can preserve the activity of the acid protease for a long time under the liquid condition.
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The infective form of schistosoma, called a cercaria, secretes proteases upon detection of human skin lipids (McKerrow & Salter, 2002).
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Kidney beans also contain protease inhibitors which frustrate the development of cancerous cells.
Green Bell Pepper stuffed with Kidney Beans
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The FDA is expected to decide soon on telaprevir, another so-called protease inhibitor from Vertex Pharmaceuticals Inc. and Johnson & Johnson .
FDA Clears Merck Hepatitis Drug
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Whey contains many types of enzymes, including hydrolases, transferases, lyases, proteases, and lipases.
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The apoptotic death signal is transmitted by caspases, a small family of proteases.
The Scientist
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ConclusionThe way of the compound neutral protease extraction is effective and reasonable.
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The aggregation of the hydrolysates of soy protein isolate(SPI) induced by subtilisin protease was investigated.
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After treatment in the HR condition, cytotrophoblast cells were lysed in a cold RIPA buffer (Pierce Biotechnology) containing a protease inhibitor cocktail (Roche).
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In order to hydrolyze the shrimp mince, different kinds of proteases have been compared respectively.
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Mast cells are also major producers of proteases.
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All of the serine proteases and the zinc peptidases of pancreatic secretions are produced in the form of their respective proenzymes.
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There is a disproportionate reduction of serous acini of the submucosal glands, which contain lysozyme, lactoferrin, and an antiprotease of small molecular weight.
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− 1 3C protease at 4°C overnight, that was subsequently dialyses into 25 mM Tris (pH
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These zinc-dependent endoproteases may act on a large number of substrates, or they may be highly specific, targeting only one or a few proteins or oligopeptides.
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AEC substrate-chromogen was purchased from Dako and cocktail protease and phosphatase solutions for protein harvest from Roche
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The combination therapy of one protease inhibitor and two nucleoside transcriptase inhibitors has markedly increased the number of CD4 cells and decreased the viral load of patients.
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Whey contains many types of enzymes, including hydrolases, transferases, lyases, proteases, and lipases.
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Objective To express the N-terminal fragment of human mannan-binding lectin(MBL) associated serine proteases-2(MASP2-N) in E.
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Late-expressed genes are, as expected, of more diverse functions and include various metabolic and structural genes, including synthases, transferases, collagen, and proteases.
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Boceprevir, a protease inhibitor, along with peginterferon and ribavirin increases response rates in previously untreated patients," said Dr. Raj Reddy, co-researcher of one of the studies and director of hepatology and medical director of liver transplantation at the University of Pennsylvania in Philadelphia.
New drug may boost hepatitis C treatment
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Ubp3 is a deubiquitination enzyme and a member of a large family of cysteine proteases that cleave ubiquitin moieties from protein substrates.
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Uroisolate PU7, was found to elicit the maximum virulence factors elaborating extracellular enzymes, protease, elastase, phospholipase C, haemolysin and pyochelin.
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This is probably due to the presence of other enzymes besides the protease.
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Besides the long-range interactions it makes with neighboring protease residues, the binding affinity of a peptide also depends on its own conformation.
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Mr. H.C. Brill [2] found raffinase, invertase, casease and protease in the pulp; oxidase, raffinase, casease and emulsinlike enzymes in the fresh bean; and all these six, together with diastase, in the fermented bean.
Cocoa and Chocolate Their History from Plantation to Consumer
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Both Incivek and Victrelis are known as protease inhibitors, designed to block an enzyme that helps the hepatitis C virus replicate.
Hepatitis C Drug Approved
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The invention discloses a composite stabilizing agent for preserving a liquid acid protease, which can preserve the activity of the acid protease for a long time under the liquid condition.
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This study was carried out to compare the handle of silk fabrics degummed with Alkalase, Protease produced by bacteria, with of silkfabrics degummed with the soap soda.
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The broad spectrum serine protease plasmin is formed following cleavage of the zymogen precursor plasminogen by host activators tissue plasminogen activator and urokinase.
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Removing CD4 from the cell surface prevents superinfection (more than one virus infecting the same cell) and helps prevent newly released viruses from turning around and infecting the same cell (also prevented by an HIV maturation step involving protease).
ERV & HIV versus Behe. Behe loses. - The Panda's Thumb
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Using techniques including atomic force microscopy and mass spectrometry, the team found that very similar enzymes, known as trypsin-like serine proteases, are at work in barnacle glue.
Signs of the Times
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This group, including trypsin and chymotrypsin, evolved from a common ancestral protease, and all use the same mechanism of action at their active site.
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The second scission occurs when a protease uses an unusual active site within the hydrophobic lipid environment to recognize and cleave the truncated target protein, releasing both the lumenal fragment and the cytoplasmic domain from the membrane.
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Proteases immobilized onto solid supports (such as agarose) are also commercially available.
The Scientist
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Degradation of fibrin clots is the function of plasmin, a serine protease that circulates as the inactive proenzyme, plasminogen.
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Fresh figs, pineapples, papayas, guavas, passion fruit, and mangoes contain an enzyme called protease, which breaks clown the protein in gelatin and prevents the liquid from setting.
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Moreover, inhibition of the cysteine protease activity by chemical inhibitors suppressed the leaf senescence process.
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They also focus on how deadly pathogens such as malaria and related parasites use rhomboid proteases to infect human cells, and how parasitic amebae use rhomboids to evade the immune system.
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Third, combined eosinophilic and neutrophilic airway inflammation may enhance eosinophilic degranulation through neutrophil protease activity.
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Treatments that have been studied but that show no clear benefit include atropine, glucagon, calcitonin and protease inhibitors.
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Both drugs are known as protease inhibitors, which are designed to block an enzyme that helps the hepatitis C virus replicate.
Hepatitis C Drugs Await FDA Approval
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Thrombolytic agents are proteases that convert plasminogen to plasmin, which subsequently lyses clot by breaking down fibrinogen and fibrin.
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Tissue plasminogen activator and, to a lesser degree, urokinase are serine proteases which convert plasminogen to plasmin.
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The viral nucleocapsid enters the host cell and breaks open releasing two viral RNA-strands and 3 essential replication enzymes: Integrase, Protease and
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Substitution of Glu 351 also altered the specificity toward non-cognate proteases: plasmin and thrombin.
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Ingredients: pure olive essential oil, gingko essence, misaiya purete, vitamin E, protease, moisturizing factor etc.
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The biochemical degradation of proteins through hydrolysis of peptide bonds is caused by the action of proteolytic enzymes or proteases.
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His team studies enzymes called rhomboid proteases that are found in a cell's membrane.
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The steady-state ITC method has been used to determine the enzyme kinetics parameters of several enzymes, including H. pylori urease, E. coli GroEL chaperonin, heparinase I, yeast hexokinase, HIV protease, pyruvate carboxylase, and dinucleoside polyphosphate hydrolase.
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Cells were placed on ice, rinsed twice with cold phosphate-buffered saline and then lysed with 10 mM Tris-HCI, pH 7.4, containing phenylmethane sulfonyl fluoride and protease inhibitor cocktail for 20 min.
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You need a supplement that provides all of the three main groups of enzymes - amylase, protease and lipase - to break down fats, carbohydrates and proteins.
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The DNA was prepared using guanidine thiocyanate, ethanol precipitation, protease K digestion, chloroform extraction and ethanol precipitation as described previously, and dissolved in TE buffer.
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If you want to selectively exclude a few bits and bobs from the cascade, what you're left with are 5 proteases, 4 of which are homologous.
Confirmation Bias and ID
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Structures of the third domain, complexed to different proteases, have been determined.
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These proteases are both endopeptidase and exopeptidase, and their combined action in the intestine leads to the production of amino acids, dipeptides, and tripeptides, all of which are taken up by enterocytes of the mucosal wall.
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In the case of the serine proteases, they are synthesized as proenzymes, larger molecules that require modification before they become active.
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A good digestive enzyme should include lipase to digests fats, amylase for carbohydrates, protease for proteins, cellulase for cellulose and fiber, and lactase for dairy.
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Prezista, which has been on the market since 2006, is a so-called protease inhibitor designed to treat HIV infection and is taken with the drug Norvir, in combination with other antiretroviral agents.
Gilead, J&J Agree to Develop New Combination HIV Drug
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Three different pathways may lead to kinin formation during inflammation: the generation of bradykinin as a result of activation of the Hageman factor and the production of plasma kallikrein; the production of lysylbradykinin by tissue kallikreins; and the action of cellular proteases in kinin formation.
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Look for a product that contains proteases (protein-digesting enzymes), such as pancreatin, papain and bromelain, and follow label directions.
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Dropout rates were similar for monotherapy and double therapy but significantly higher in trials containing protease inhibitors.
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The number of proteases, peptidases, and other exoenzymes produced by S. miilans clearly suggests that it derives resources from host tissues.
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In one investigation, Schmitt found that enzymes called serine-class proteases, which break down proteins in the sprouting grain, can also break down beta-amylase, an important enzyme for converting carbs to simple sugars.
Daily News & Analysis
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PMSF completely inhibited the fibrinolytic activity, but EDTA and EGTA exerted little effect on it. These results demonstrate that DFE is a serine protease.
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Proteases are a class of hydrolytic enzymes mediating irreversible disruption of protein amide bonds.
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Researchers have used this approach to identify protease and kinase substrates, protein: protein interactions, and protein: nucleic acid interactions.
The Scientist
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Cell extracts were obtained with RIPA buffer in the presence of protease inhibitors (sodium pyrophosphate, sodium fluoride, phenylmethanesulfonyl fluoride, pepstatin A, aprotinin, leupeptin, antipain, benzamidine - all from Sigma).
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Other categories include transferases, oxidoreductases, ligases, lyases, isomerases, proteases, molecular chaperons, and GTPases.
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For instance, the set of enzymes known as serine proteases are protein-digesting enzymes in the intestine.
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Both the Merck and Vertex drugs are known as protease inhibitors, which are designed to block an enzyme that helps hepatitis C virus replicate.
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A good digestive enzyme should include lipase to digests fats, amylase for carbohydrates, protease for proteins, cellulase for cellulose and fiber, and lactase for dairy.
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The transient activation of bone resorption function, presented as the temporary increase of osteoclasts number and mostly maintenance of its function of excreting acid and protease.
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Thus we sought to assess the role of cell permeable inhibitors of cysteine proteases; caspase (zVADfmk) and calpain (zLLYfmk) as supplements in the serum free culture of CD34
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Although starches and fats can be broken down without pancreatic enzymes, pancreatic proteases are crucial for protein digestion.
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The completion of the sequence depended on the use of specific proteases - trypsin, chymotrypsin, and pepsin - to produce larger fragments.
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These death proteases are part of a proteolytic cascade activating proenzymes into active proteases.
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The combination is commonly known as a protease cocktail.
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Standard doses of garlic caplets can cut blood levels of the protease inhibitor saquinavir (brand name Crixivan) by 50 percent, according to a study conducted by the National Institute of Allergy and Infectious Diseases.
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Chapter 5 focuses on the catabolism of proteoglycans, detailing mainly proteolysis and the host of proteases involved.
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Protease samples were submitted to electrophoresis in polyacrylamide gels under denaturing conditions, according to Laemmli.
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Rennin is an acid protease, meaning it breaks down protein bonds in an acidic environment.
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As an important leucoprotease, myeloperoxidase (MPO) has many effects in mediating inflammation, regulating immune response and so on, which also takes part in the genesis and development of diseases.
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Talin binds vinculin and integrins. talin is a substrate for the Ca2+-activated protease calpain II.
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The viral nucleocapsid enters the host cell and breaks open releasing two viral RNA-strands and 3 essential replication enzymes: Integrase, Protease and Reverse Transcriptase sterling silver cubic zirconia three strand pearl bracelet
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Trypsin also activates trypsinogen as well as all the other proenzymes in the pancreatic secretion, producing the active proteases and peptidases that hydrolyze dietary polypeptides.
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(Dang QD, Di Cera E Residue 225 determines the Na (+) - induced allosteric regulation of catalytic activity in serine proteases.
Protein-protein Interactions
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Abbott labs has begun testing a protease inhibitor designed to be five times more powerful than those now available.
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A holy between protease-inhibitor zona and these conflicts has yetabdominally overlayed established and the long-term steadies are constently unknown.
Wii-volution
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Objective To investigate the role of protease-antiprotease imbalance in pulmonary contusion after the chest impact trauma in the rabbits.
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Tissue plasminogen activator and, to a lesser degree, urokinase are serine proteases which convert plasminogen to plasmin.
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The hydrolysis of peanut meal using neutral protease was investigated.
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Following removal of the protease the cells were shifted to 37°C in the presence of cycloheximide.
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Proprotein convertases (PC) are a family of subtilisin-like serine proteases.
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In order to improve the solubility of isolated and concentrated soybean protein, the hydrolysis of those proteins was controled restrictively using basic protease and neutral protease.
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Mr. H.C. Brill [2] found raffinase, invertase, casease and protease in the pulp; oxidase, raffinase, casease and emulsinlike enzymes in the fresh bean; and all these six, together with diastase, in the fermented bean.
Cocoa and Chocolate Their History from Plantation to Consumer
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Snake venom thrombin - like enzymes belong to the fibrinogenolytic enzyme family and are serine proteases.
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The proenzyme structure reveals suboptimal catalytic triad geometry with its propiece linker partially blocking the substrate-binding site, which together serve to prevent premature activation of the protease.
Journal of Biological Chemistry current issue
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Cells were washed 3 times in PBS, and detached from the plate surface with cell scrapers in the presence of protease inhibitors (sodium pyrophosphate, sodium fluoride, phenylmethanesulfonyl fluoride, pepstatin A, aprotinin, leupeptin, antipain, benzamidine - all from Sigma) diluted in PBS.
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- 1995: Two new classes of anti-HIV drugs, also targeting replication, are approved: protease inhibitors and non-nucleoside reverse transcriptase inhibitors.
ANC Daily News Briefing
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There is, however, growing evidence that specific proteases may also act as mediators of signal transduction or effectors of programmed cell death during plant senescence.
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The expression of this gene is associated with concomitant changes in cysteine protease activity of the petals.
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Acetes Chiensis was hydrolyzed by alkalic protease, and shrimp sauce was produced with disinfecting and packing.
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The proteases are secreted in forms of zymogen (ie, inactive precursor to enzyme), such as trypsinogen, chymotrypsinogen, and procarboxypeptidase.
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There are many bacterial virulence factors that may contribute to clinical outcomes, including bacterial hemolysins, proteases, and siderophores.
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Whey contains many types of enzymes, including hydrolases, transferases, lyases, proteases, and lipases.
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In chordates, collectins initiate the lectin cascade through members of the mannose-binding protein (MBP)—associated protease (MASP)/C1r/C1s family.
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The role of two cysteine proteases; caspase and calpain has been highly implicated in programmed cell death in many cell systems
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Cells were placed on ice, rinsed twice with cold phosphate-buffered saline and then lysed with 10 mM Tris-HCI, pH 7.4, containing phenylmethane sulfonyl fluoride and protease inhibitor cocktail for 20 min.
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Nakayama K (1997) Furin: a mammalian subtilisin/Kex2p-like endoprotease involved in processing of a wide variety of precursor proteins.
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CWD infection was confirmed in all cases by the presence of histologic lesions in the brain including spongiform degeneration of the perikaryon, the immunohistochemical detection of disease-associated PrP in brain and tonsil, or by immunoblotting of protease-resistant, disease associated PrP (CerPrPSc).
Undefined
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The oxidative stress can begin a cascade of events leading to cell shrinkage, DNA fragmentation, and activation of protease.
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Again, they used partial acid hydrolysis of the whole chain and then protease digestion to give larger blocks.
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Acid also kills germs in food small intestine here pancreatic juice (containing (duodenum and carbohydrase, protease, lipase)
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