How To Use Supernatant In A Sentence
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The suspensions were spun at 1000 g for 30 minutes and the supernatants were filtered and lyophilized.
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All extracts were centrifuged at 10000 rpm for 5 min, and the supernatants were then evaporated under vacuum to dryness and resuspended in appropriate solvents to a final concentration of 1 mg/ml.
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The supernatant was removed and stored and the pellets extracted with 100% acetone until they were colourless.
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The culture supernatant could stimulate the neurite outgrowth of PC12 cells.
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Supernatant fractions were removed and TCA precipitated as described above, and pellet fractions were resuspended in solubilization buffer.
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The supernatant was collected and the sediment suspended in a double volume of water, acidified and centrifuged.
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Ten minutes after adding malate, we again pipetted the supernatant from the same dish into other test tubes.
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The supernatant and the precipitate fractions were separated.
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The supernatants were used for assays of enzyme activity.
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Methods Observing the levels of parasitemia in mice, development of sexual stage parasites in mosquitoes and induced effect of PRBC extract on NO 2 -from supernatants of spleen cells.
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The supernatant was assayed for the determination of glycerol as just described for triglycerides.
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The supernatant from the first centrifugation was centrifuged at 10,000 × g for 10 min.
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Lysates were cleared by centrifugation for 20 minutes at 15,000 rpm in a microcentrifuge and the supernatants were assayed for protein concentration by Bradford assay using BSA as a standard.
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Cell debris was pelleted by centrifugation and the supernatant was tested for protein induction.
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Then, the eluate was diluted to a final concentration of 0.1% SDS with lysis buffer and immunoprecipitation was repeated with the supernatant with fresh beads and antibody.
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Cell debris was pelleted by centrifugation and supernatant concentrated by lyophilization to one tenth of the initial volume.
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After 1 min of extraction, cells were sedimented and the supernatant was decanted.
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This supernatant was used for measurement of isotopic enrichment of free leucine.
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Supernatant fractions were precipitated with trichloroacetic acid, washed with acetone, and resuspended in ribosome solubilization buffer.
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At steady state, cells were centrifuged and the fluorescence of the supernatant measured.
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Agglutination reaction was carried out using the reagents with the stool supernatants.
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The supernatant was precipitated by isopropanol.
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Liquid black ink, 25 c.c. Tincture of iodine 1 c.c. Allow the mixture to stand 24 hours, centrifugalise thoroughly, pipette off the supernatant liquid to a clean bottle and then add a crystal of thymol or one drop of formalin as a preservative.
The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
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The supernatant was desalted by centrifugal gel filtration and concentrated by ultrafiltration as previously described.
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Fluorescence experiments after centrifugation confirmed that there were no vesicles in the supernatant.
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The sonicate was centrifuged for 10 min at 16,000 RCF and the resulting supernatant collected.
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The samples were subject to two cycles of freezing/thawing and low molecular weight thiols were recovered in the supernatants after centrifugation at 12 000 g, 10 min at room temperature.
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The precipitate formed is allowed to settle, 50 c.c. of the supernatant solution is removed with a pipette and transferred to a beaker; 50 c.c. of decinormal nitric acid and some water is added with sufficient cochineal tincture.
Scientific American Supplement, No. 324, March 18, 1882
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Supernatants were subjected to ultracentrifugation in a Beckman SW28 rotor at
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The culture supernatants, though produced cytopathic effect in various tissue cultures, failed to have any deleterious effect on the worms.
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The concentration of unbound protein in the supernatant was determined spectrophotometrically at 410 nm.
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The supernatant was desalted by centrifugal gel filtration and concentrated by ultrafiltration as previously described.
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The 10 ml samples were centrifuged and the absorbance of the supernatant was measured at 280 nm to determine the lignin content.
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Cell debris was removed by centrifugation, and the supernatant was fractionated by ammonium sulfate precipitation.
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The supernatant was either dialyzed against sterile water (for elastase activity) or sterile filtered using 0.2 µm pore filters (16543; Sartorius), frozen and kept for further analysis.
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The supernatant was used for soluble sugar determination and the last pellet was redissolved in distilled water for starch determination.
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The clear supernatant was loaded onto a Ni-chelating column, and bound protein was eluted with 500 mM imidazole.
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Acid insoluble material was pelleted by centrifugation at 14,000 x g for 10 min and the supernatant was mixed with 1/4 volume 100% trichloroacetic acid and incubated on ice for 1 hr.
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The erythrocytes were allowed to settle for 1 h, and the plasma, leukocytes and thrombocytes were separated by aspirating the supernatant.
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The ammoniacal acetone supernatant containing extracted pigments was discarded, and the lipoprotein pellet was suspended in 2 mL of homogenization buffer.
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After various time intervals the supernatants were removed, cell samples were fixed with paraformaldehyde solution for 30 minutes at room temperature.
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This pellet was utilized to prepare bacteriorhodopsin while the supernatant was used for the isolation of crude fatty acids.
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The existence of such an RNase inhibitor (RI) was first inferred in 1952 from the decrease in RNase activity in liver supernatants, and it exists in almost all mammals.
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This can be counteracted by precipitating in both solutions a mixture of ferric and aluminic hydrates, which settles readily and leaves the supernatant liquor clear.
A Text-book of Assaying: For the Use of Those Connected with Mines.
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Samples were then centrifuged (17,000 × g for 10 min), and supernatant fluid was stored at - 20°C for VFA analysis.
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The supernatant was recentrifuged at 109,000 g on a tabletop ultracentrifuge (Rotor TLA 45 of Beckman centrifuges, Beckman Coulter, Fullerton, CA) for 1 h.
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Finally, the excess lipid was eliminated by centrifuging the sample for 1 min, discarding the supernatant, rehydrating and vortexing the sample five times.
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ZNF9 and GAPDH western blotting of supernatant and polysome fractions of HeLa cell lysates isolated in the absence or presence of EDTA.
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Again remove the supernatant fluid and fill in a fresh supply of saline solution and centrifugalise once more.
The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
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Material was spun at 6000 rpm for 1 min to pellet nucleic acid, and supernatant was removed to a clean tube.
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The supernatant was taken for spectrophotometric measurements.
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Objective : To study the growth factor like action of the Sika Deer splenocytes culture supernatant.
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The homogenates were then centrifuged at 3000 rpm for 15 min and the supernatants were used for determination of the lipid peroxide level via the MDA method, as described above.
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Samples were centrifuged for 2 min and the supernatant aliquoted and stored at - 80'C until use.
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DNA was precipitated from the supernatant using isopropanol and exposed to two 70% ethanol washes.
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The tubes were centrifuged at 5000 rpm for 2 min, and the supernatant discarded.
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The supernatant was discarded and the pellets were dried in a desiccator over silica gel for 24 h and then extracted for the various assays.
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Confirmation of chromophore adsorption to spheres was done by centrifuging chromophore-sphere preparations and remeasuring absorption and fluorescence spectra of the supernatant.
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After removing the cell debris by centrifugation, supernatant was layered on a 5.7 M CsCl / 0.01 M EDTA solution in an ultracentrifuge tube and centrifuged in a swinging bucket rotor at 40,000 rpm for 16 hr.
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The supernatants were removed and the pellets triturated with 700 l of PBS / 10% FBS.
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The supernatant was kept in stock whereas the residue was sequentially extracted three times.
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the supernatant fat was skimmed off
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The supernatant fluid was filtered and centrifuged.
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The amount of haemolysis was measured by comparing the absorption of the blood to that of the supernatant after sedimentation of the red cells.
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At steady state, cells were centrifuged and the fluorescence of the supernatant measured.
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Cell debris was removed by centrifugation, and the supernatant was fractionated by ammonium sulfate precipitation.
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Qualitatively, no difference was observed in the cytotoxicity of unheated, heated and ultra filtered culture supernatants of isolates.
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The total lysate separated into 4 different fractions whereas only the one with the lowest density was detected when the supernatant recovered after ultracentrifugation was loaded onto the sucrose gradient.
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After the medium and bacteria were centrifuged for 10 min at 6000 g, the supernatant containing the exoenzymes was collected and stored at -20°C until use.
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Fluorescence of the lysed cellular supernatants in the ApoAlert caspase profiling plates was read in a microplate fluorometer, by use of excitation and emission wavelengths of 380 and
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Absolute alcohol 60 c.c. Allow to stand twenty-four hours, then centrifugalise thoroughly and decant the supernatant fluid into a well-stoppered bottle.
The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
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The supernatant was ultracentrifuged at 105,000 × g, 4°C for 1.5 h.
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These were shaken for 1 hour, then the solutions were centrifuged and the clear supernatant was pipetted into vials.
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The resultant supernatant fraction was used for the determination of lipid peroxidation and glutathione.
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After incubation, the samples were treated as above, and the supernatant fractions were lyophilized.
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Ammonia N was determined on the supernatant fluid using the phenol-hypochlorite procedure.
