How To Use Transposon In A Sentence
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The researchers constructed the transposons in such a way that they can carry the therapeutic gene into the target cell DNA.
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The DNA transposons resemble typical bacterial transposons, they have terminal inverted repeats which enclose the transposase gene.
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In DNA footprinting analyses using the C-module of retrotransposon TRE5-A as probe, two CbfA binding sites have been determined that contain 14-22 homopolymeric thymidines
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When retrotransposons are inserted into important genes, they can cause disease, such as hemophilia and muscular dystrophy.
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When retrotransposons are inserted into important genes, they can cause disease, such as hemophilia and muscular dystrophy.
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Seven insertions were located in the retrotransposon array, indicating that the terminal array is not refractory to P-element integrations.
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The high copy number and general dispersion of retrotransposons throughout the genome, as well as the large local change caused by their insertion, provide an excellent basis for the development of DNA-based markers.
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Its genome size might have increased as assumed, decreased or remained the same subject only to genomic turnover, with new retrotransposon families replacing old ones or other secondary DNA.
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The transposon sequence is represented between backslashes.
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One of the mechanisms for the movement of genes is the so-called retrotransposable element, or retrotransposon.
Human Evolution: It's all in the testes - The Panda's Thumb
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To me however, the most interesting components of junk DNA are the mobile genetic elements, also called jumping genes or transposons.
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Kalendar et al. found that the number of copies of a long terminal repeat retrotransposon BARE - 1, that is present in all Hordeum species, was clearly correlated with altitude in the canyon.
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A transposon is a segment of DNA that can replicate itself and move around to different positions within the genome.
The Times of India
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Rapid amplification of a retrotransposon subfamily is evolving the mouse genome.
Parasite Rex
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Transcriptional disruption by the L1 retrotransposon and implications for mammalian transcriptomes.
The Edge of Evolution
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Several DNA sequences that show sequence similarity to transposons were isolated from these sex chromosomes.
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The work also revealed that ZBED6 is a "transposon" - a piece of DNA that inserted into the genome at some point in evolution.
PhysOrg.com - latest science and technology news stories
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Position-effect variegation results from the juxtaposition of euchromatin and heterochromatin by chromosome rearrangement or transposon insertion.
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Transposons are just small pieces of DNA that randomly insert in the genetic code.
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It has been proposed that in transposon-containing regions of the genome both DNA strands are transcribed, dsRNA is formed, and the RNAi process eliminates these undesirable products.
Advanced Information: The 2006 Nobel Prize in Physiology or Medicine
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This could occur during the migration of a retrotransposon, or when a virus incompletely inserts its genome into the cellular genome.
Dembski versus Europe - The Panda's Thumb
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Transposon system is easy to manipulate, quick and can achieve high throughput, carry multiple resistance marker gene, which makes simultaneous study of multiple genes possible.
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This suggests that the insertion could affect either the transcriptional or the transpositional activity of the retrotransposons or the epigenetic state of that chromosomal region.
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In the phylogenetic tree of TERT and retroelements rooted with RNA-dependent RNA polymerases, the RT motifs in the RT family are universally conserved [including multicopy single-stranded DNA, group II introns, LTR or non-LTR (Long Terminal Repeat) retrotransposons, and viral RT] [10], [89].
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Nucleotide sequence changes in the two termini of individual retrotransposons were used to date their time of insertion.
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amplifying" the stretches of individual genomes that border transposons and reading thousands of the junctions with advanced sequencing techniques, then comparing them to the reference human genome.
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We hypothesized that the highly efficient plasmid-based piggyBac transposon system would enable long-term inducible gene expression in mice in vivo.
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His nonviral method uses a jumping gene, called a transposon, to insert therapeutic genes into target cells.
Starbulletin Headlines
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In fact, in evolutionary terms, it is likely that such sequences have been more successful as transposons than as host genes.
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The transposon is usually a gene that codes for a protein.
Immune System Evolution #1: Comments on Annotated Bibliography - The Panda's Thumb
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Molecular access to the sda gene was via the sda HZ.P1 mutation by the method of plasmid rescue of genomic DNA flanking the transposon insertion.
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More recently, specific subsets of repetitive DNA, such as retrotransposons, have been shown to comprise a high percentage of the repetitive DNA of plants.
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We performed PCR on wild-type mouse genomic DNA to amplify sequences flanking transposon insertion sites for use as probes.
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In the case of T-DNA and some transposon insertions there is no realistic possibility of reversion to wild type.
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Most impressive was the prediction that a free-living transposon homologous to RAG would exist.
Assessing Applegate's Attack
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At these positions, although the trigger works fine, and the transposon is silenced, once the trigger is lost, the transposon reawakens, said Jaswinder Singh, a professor in the Plant Sciences Department at McGill University, and lead author of the new article.
Can't We All Be Friends?
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3 Several “junk DNA” features are shared with Chimpanzees and other primates, such as retroviral transposons inserted into specific genes.
Another unintelligent move - The Panda's Thumb
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This evidence came in only in 2006, but there is actual direct evidence that the transposon-derived ancestors of the RAG genes were in deuterostome genomes * before* they were used to generate receptor diversity.
Assessing Applegate's Attack
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I'd also point out that the delectable S A Smith overeggs her cake by claiming, 'retroelements are just dandy for phylogenetic analysis', what the paper she cites actually says is, 'The presence of a retrotransposon at a single locus in multiple taxa remains an extremely powerful phylogenetic marker, but caution is required before concluding that the existence of a particular SINE at a particular locus in multiple individuals is indicative of common ancestry'.
On Thursday, the Legg report will be published along with...
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Thus we were able to clone the DNA sequence that flanked the transposon insertion using plasmid rescue.
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A single injection of piggyBac transposons could achieve long-term inducible gene expression in the livers of mice in vivo, confirming our multiple-transposon strategy used in cultured cells.
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So the thing which you think is most unlikely, the sudden incorporation of the transposon from a bacterium, right into the right spot in the right receptor in some early fish genome, didn't have to happen all in one step, indeed we now have evidence that it did not.
Assessing Applegate's Attack
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Similar arguments and requirements apply to mutants created by transposon mutagenesis.
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While transposon mutagenesis involves elaborate PCR screening, RNAi generates only gene-specific phenocopies of loss-of-function mutations and does not always cause a true null phenotype.
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These results support the hypothesis that transposons accumulate in nonrecombining regions and may be one cause of the heteromorphism of sex chromosomes.
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Distribution and transposition of endogenous retrotransposon sequences may contribute to the organization of genome structure and evolution, but this remains purely speculative.
