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counterstain

NOUN
  1. a stain of contrasting color that is used when the principal stain does not show the structure clearly

How To Use counterstain In A Sentence

  • The sections were then counterstained in eosin, dehydrated, cleared, and mounted with glass coverslips.
  • Following incubation with fluorescence-conjugated secondary antibody (at room temperature for 60 min) and rinses, slides were incubated with DAPI (1: 10,000, Sigma, St. Louis, MO) for 15 minutes to counterstain nuclei, mounted, and cover-slipped. PLoS ONE Alerts: New Articles
  • The chromosomes are counterstained with DAPI (light blue).
  • Harris hematoxylin was the counterstain for the remainder of the antibody reactions.
  • After washing in tap water, specimens were briefly counterstained.
  • α (brown; g; nuclear counterstain is methyl green), Ttf1 Naturejobs - All Jobs
  • The sections were counterstained with hematoxylin and eosin.
  • Bottom: reelin immunofluorescence (red) overlaid with MAP2 counterstain PLoS ONE Alerts: New Articles
  • After washing, the HRP (horseradish peroxidase) conjugated polymer complex was visualized by incubation for ~5 min with AEC (3-amino-9-ethylcarbazole) substrate-chromogen; sections were then counterstained with hematoxylin for 30 seconds, washed and mounted. PLoS ONE Alerts: New Articles
  • The shape of the nucleus was visualized using a DNA counterstain in all experiments.
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